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Bipyridine (bpy), recognized as an effective ligand for Ni II , 253 was first attached covalently to the PSP2-Cys95 mutant, resulting in the formation of PSP-95C-bpy, followed by complexing with Ni II to produce the photosensitizer metalloenzyme PSP-95C–Ni II (bpy), which consequently integrates a genetically encoded BpA and a site-specifically modified synthetic Ni II (bpy) cofactor
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