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Nonspecific binding sites were blocked with either non-fat dry milk or BSA, before overnight incubation in protein-specific primary antibodies to ACE2 (Proteintech, 66699-1, accession number: BC048094), ICAM-1 (Proteintech, 60299-1, accession number: BC015969), PAI-1 (Santa Cruz, 8979, accession number: P05121), phospho-ERK1/2 (Cell Signalling, 9101S, accession number: P27361, P28482), ERK1/2 (Santa Cruz, 514302, accession number: P27361, P28482), phospho-NFκB (Cell Signalling, 3033, accession number: Q04206), NFκB (Cell Signalling, 6956, accession number: Q04206), phospho-eNOS Ser1177 (Cell Signalling, 9571, accession number: P29474) and eNOS (Cell Signalling, 9572, accession number: P29474)

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