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After treatment, cells were washed once with 1xPBS, and then incubated/stained with anti-Thy1.1-APC (1:400, BioLegend, 202526), Zombie NIR™ Fixable Viability Dye (1:1000, BioLegend, 423105), and Human TruStain FcX™ Fc Receptor Blocking Solution (1:400, BioLegend, 422301) in FACS buffer (1xPBS, 5% FBS, 1 mM EDTA) for 10 min at 4°C

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Toxins 10:155