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All samples were run in triplicates, and relative quantification (RQ) was calculated following the ΔCt method: RQ = 2 −ΔCt , where ΔCt is the difference between the Ct of the gene of interest and the Ct of the endogenous gene control β-actin

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264 In summary, the beneficial effects of TZDs on the improvement and even the resolution of steatosis and histologic features of MASH were highly consistent, but the effects on liver fibrosis were inconsistent with several studies suggesting no significant improvement in fibrosis with TZDs, possibly because the intervention time was not long enough to produce significant improvement in fibrosis or because of the differences in baseline fibrosis stage between studies