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Lencer (Boston Children’s Hospital, Harvard Medical School and Harvard Digestive Diseases center, United States) were cultured in MCDB 131 medium (Gibco) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Gibco), 2 mM l-glutamine (Gibco), 25 µg/mL streptomycin (Gibco), 25 U/mL penicillin (Gibco), 10 ng/mL recombinant mouse epidermal growth factor (Gibco), and 1 µg/mL hydrocortisone (Sigma-Aldrich), at 37 °C in a humidified atmosphere of 5% CO 2

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After euthanasia, the knee joints were surgically exposed, and the femoral and tibial condyles were examined for gross morphological changes

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