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Participants reported that they were not taking any medication and were instructed to abstain from the use of alcohol, nicotine, recreational drugs, and foods or beverages containing caffeine for three weeks prior to the study
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Assessing the tissue ET concentrations of control mice, we found that of the 10 tissues measured (expressed in mass per wet weight of tissue ± standard error), liver had the highest amount of ET (80.65 ± 4.14 ng/mg), followed by whole blood (58.99 ± 2.05 ng/µl blood), spleen (36.45 ± 3.72 ng/mg), kidney (27.61 ± 2.61 ng/mg), lung (19.56 ± 1.46 ng/mg), heart (12.09 ± 1.20 ng/mg), small intestine (7.23 ± 0.86 ng/mg), eye (5.40 ± 0.29 ng/mg), large intestine (4.37 ± 0.93 ng/mg) and brain (3.73 ± 0.59 ng/mg) (Fig