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Structural configuration is critical, with linear polyamines outperforming branched, T-shaped, or globular variants in NA condensation and transfection [89] with DOGS, inspiring spermine-based vector designs [87]

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M1 macrophages are mainly induced by T helper type 1 (Th1) cytokines such as interferon γ (IFN-γ), Toll-like receptor (TLR) stimulation with agonists like lipopolysaccharide (LPS), and granulocyte-macrophage colony-stimulating factor (GM-CSF) (43, 44)