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These metals can catalyze the Fenton reaction [86], where they interact with hydrogen peroxide to generate reactive hydroxyl radicals, one of the most reactive forms of ROS, leading to oxidative damage of sperm cells and their DNA damage [78]

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ROS/RNS induce irreversible protein modifications, such as carbonylation or nitration, leading to permanent loss of the functions of proteins as enzymes, receptors, and transporters [71]