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Injection of rGDF11 rapidly activates SMAD signaling in cardiac tissue of both young and old mice 104 , which together indicate that exogenous GDF11 can regulate cardiomyocyte size and hypertrophy and highlight that doses and protein preparations used could impact the results of in vivo studies 105

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The rationale for combining these peptides lies in the hypothesis that parallel modulation of nitric oxide signaling, cytoskeletal dynamics, extracellular matrix remodeling, and oxidative stress responses may produce additive or synergistic effects in controlled research models